G-Matrix Equation in the Resonating-Group Method

The G-matrix equation is most straightforwardly formulated in the resonating-group method if the quark-exchange kernel is directly used as the driving term for the infinite sum of all the ladder diagrams. The inherent energy-dependence involved in the exchange term of the normalization kernel plays the essential role to define the off-shell T-matrix uniquely when the complete Pauli-forbidden state exists. We analyze this using a simple solvable model with no quark-quark interaction, and calculating the most general T-matrix in the formulation developed by Noyes and Kowalski. This formulation gives a certain condition for the existence of the solution in the Lippmann-Schwinger resonating-group method. A new procedure to deal with the corrections for the reduced masses and the internal-energy terms in the Lambda N - Sigma N coupled-channel resonating-group equation is proposed.Comment: 21 pages 0 figures, submitted to Prog. Theor. Phy

The Brieva-Rook Localization of the Microscopic Nucleon-Nucleus Potential

The nonlocality of the microscopic nucleon-nucleus optical potential is commonly localized by the Brieva-Rook approximation. The validity of the localization is tested for the proton+$^{90}$Zr scattering at the incident energies from 65 MeV to 800 MeV. The localization is valid in the wide incident-energy range.Comment: 20 pages, 8 figure

A Novel Method for Screening Monoclonal Antibodies Reacting with Antigenic Determinants on Soluble Antigens; A Reversed Indirect-Enzyme Linked Immunosorbent Assay(RI-ELISA)

A novel screening method was established to select new monoclonal antibodies which react with unknown antigenic determinants on molecules bearing antigen determinants reactive with established monoclonal antibodies. This new method is a sandwich assay termed "reversed indirect-enzyme linked immunosorbent assay" (RI-ELISA). Goat antimouse immunoglobulin antibodies are used as the primary immobilized antibody in this assay. They allow the non-purified monoclonal antibodies contained in hybridoma culture supernatants to bind to the microtest plate for enzyme immunoassay (EIA plate) much more efficiently than in the usual sandwich assay where the non-purified monoclonal antibodies are adsorbed directly to the polystyrene surface. The antigen solution is then reacted with the monoclonal antibodies and thereafter enzyme labeled monoclonal antibody with known specificity is added. Therefore, if the hybridoma culture supernatant contains monoclonal antibodies which were bound to the EIA plate and react with antigenic determinants on the soluble molecules which have antigen determinants recognized by the enzyme labeled antibody, the enzyme labeled antibodies will bind to induce an enzymatic reaction. The most important technical consideration in the RI-ELISA is the inhibition of direct binding of the enzyme labeled monoclonal antibodies to free sites remaining in the immobilized goat anti-mouse immunoglobulins antibodies. This problem could be effectively overcome by using normal mouse serum as blocking substance. These studies indicate that the RI-ELISA may be a useful screening method for selecting new monoclonal antibodies which react with unknown antigenic determinants on soluble molecules

Comparison of Various Serum Protein Values in the Japanese and the Japanese-Americans Resident in the United States

Measurements were made of various types of proteins, that is α1-antitrypsin, α1-acid glycoprotein, α2-HS glycoprotein, haptoglobin, α2-macroglobulin, transferrin, C3, IgG, IgA and lgM, in the serum of the Japanese-Americans living in Hawaii and the Japanese-Americans living in Los Angeles who are assumed to be genetically almost identical to the Japanese in Hiroshima Prefecture but are known to have a higher intake of animal fats but a lower intake of complex carbohydrates. These were compared with those of the Japanese in Hiroshima Prefecture. α2-macroglobulin values in serum of the male Japanese-Americans living in Hawaii of ages 30-39 years, 40-49 years, and 50-59 years were significantly lower than those of the residents in Hiroshima Prefecture, but no significant difference in these values could be observed between the Japanese-Americans living in Los Angeles and the Japanese in Hiroshima Prefecture. No significant difference could be observed in the values of other serum proteins in all age groups. These findings indicate that the difference in intake volume of animal fats and complex carbohydrates did not affect these serum protein values